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 Carluke Rovers v Carluke Rovers U20s
 Pre-Season

 Saturday 22nd May 2021

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 Newmains United 3 v 1 Carluke Rovers
 Pre-Season Breathing Space Cup

 Saturday 29th May 2021

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 Carluke Rovers 5 v 1 Royal Albert
 Pre-Season Breathing Space Cup

 Saturday 5th June 2021

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 Carluke Rovers 2 v 2 Forth Wanderers
 Pre-Season Breathing Space Cup

 Saturday 12th June 2021

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 Carluke Rovers 1 v 4 Bellshill Athletic
 Pre-Season Breathing Space Cup

 Thursday 17th June 2021

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 Lesmahagow Juniors 0 v 0 Carluke Rovers
 Pre-Season Breathing Space Cup

 Saturday 26th June 2021

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 Mid Annandale 1 v 6 Carluke Rovers
 Pre-Season Friendly

 Saturday 10th July 2021

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 Nithsdale Wanderers 2 v 3 Carluke Rovers
 Pre-Season Friendly

 Saturday 17th July 2021

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 Gartcairn 6 v 0 Carluke Rovers
 WOSFL Conference B

 Wednesday 21st July 2021

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 Carluke Rovers 4 v 0 Royal Albert
 WOSFL Conference B

 Saturday 24th July 2021

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 Thorniewood United 1 v 1 Carluke Rovers
 WOSFL Conference B

 Wednesday 28th July 2021

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 Carluke Rovers 1 v 0 Kello Rovers
 WOSFL Conference B

 Saturday 31st July 2021

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 Carluke Rovers 3 v 5 Ardeer Thistle
 WOSFL Conference B

 Wednesday 4th August 2021

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 Port Glasgow Juniors 3 v 1 Carluke Rovers
 WOSFL Conference B

 Saturday 7th August 2021

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 Carluke Rovers 1 v 4 St Cadocs
 WOSFL Conference B

 Wednesday 11th August 2021

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 Glasgow United 3 v 1 Carluke Rovers
 WOSFL Conference B

 Saturday 14th August 2021

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 Carluke Rovers 2 v 3 Ashfield
 WOSFL Conference B

 Wednesday 18th August 2021

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 Carluke Rovers 3 v 1 Sunnybank
 Scottish Junior Cup

 Saturday 21st August 2021

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 Carluke Rovers 0 v 8 Greenock Juniors
 WOSFL Conference B

 Saturday 28th August 2021

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 Dalry Thistle 1 v 2 Carluke Rovers
 WOSFL Conference B

 Saturday 4th September 2021

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 Carluke Rovers 1 v 6 Cambuslang Rangers
 South Challenge Cup

 Saturday 11th September 2021

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 Renfrew 2 v 4 Carluke Rovers
 WOSFL Conference B

 Saturday 18th September 2021

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 Carluke Rovers 1 v 2 Auchinleck Talbot
 Scottish Junior Cup Round 2

 Saturday 25th September 2021

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 Carluke Rovers 5 v 4 Glasgow United
 WOSFL Conference B

 Saturday 2nd October 2021

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 Cambuslang Rangers 8 v 0 Carluke Rovers
 WOSFL Conference B

 Saturday 9th October 2021

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 Carluke Rovers 1 v 2 Newmains United
 WOSFL Conference B

 Tuesday 12th October 2021

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 Carluke Rovers 1 v 2 Dalry Thistle
 WOSFL Conference B

 Saturday 16th October 2021